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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Interleukin 4 Moderately Affects Competence of Pluripotent Stem Cells for Myogenic Conversion
doi: 10.3390/ijms20163932
Figure Lengend Snippet: Analysis of expression of IL-4 and IL-4 type II receptor subunits in undifferentiated mouse ESCs and mouse primary myoblasts. ( A ) Expression of Il-4rα and Il-13rα1 in undifferentiated ESCs and primary myoblasts analyzed at four stages of the culture: M0 (freshly isolated satellite cells), M3 (day 3 of the culture, predominately proliferating myoblasts), M6 (day 6 of the culture, predominately fusing myoblasts), and M9 (day 9 of the culture, predominately myotubes). β-actin was used as a reference gene. RQ = 1 for the level of gene expression detected in 13.5-day-old mouse embryo. Data are presented as the means of three independent experiments with standard deviations; ** p < 0.01. ( B ) Representative images of immunocytochemistry analysis of IL-4Rα, IL-13Rα1, and IL-4 in undifferentiated ESCs. Scale bar: 20 μm. ( C ) Representative images of immunocytochemistry analysis of IL-4Rα, IL-13Rα1, and IL-4 in mouse primary myoblasts at day 6 (white arrows indicate myoblasts, grey arrows indicate myotubes). Scale bar: 20 μm.
Article Snippet: Additionally, ESCs and EBs were cultured in the presence of 5 ng/mL IL-4 and either 5 μg/mL or 10 μg/mL of anti-IL-4 antibody (I7034 Sigma Aldrich), or 5 ng/mL or 10 ng/mL of
Techniques: Expressing, Isolation, Gene Expression, Immunocytochemistry
Journal: International Journal of Molecular Sciences
Article Title: Interleukin 4 Moderately Affects Competence of Pluripotent Stem Cells for Myogenic Conversion
doi: 10.3390/ijms20163932
Figure Lengend Snippet: Analysis of expression of IL-4 and IL-4 type II receptor in ESCs differentiating in the EBs. ( A ) Expression of Il-4rα and Il-13rα1 in undifferentiated ESCs, ESCs differentiating in EBs for 2 (EB2), 5 (EB5) and 7 (EB7) days, and proliferating C2C12 myoblasts at day 3 of the culture (M3). β-actin was used as a reference gene. RQ = 1 for the expression level detected in 13.5-day-old mouse embryo. Data are represented as the means of three independent experiments with standard deviations; * p < 0.05; ** p < 0.01. ( B ) Percentage of undifferentiated ESCs, cells obtained after disaggregation of EB2, EB5, and EB7 and proliferating C2C12 myoblasts synthesizing IL-4Rα or IL-13Rα1, analyzed with flow cytometry. * p < 0.05. ( C , D ) Representative histograms presenting percentage of cells synthesizing IL-4Rα ( C ) or IL-13Rα1 ( D ) in unstained ESCs (negative control, upper) and stained populations of either ESCs, EB2-derived cells, and C2C12 myoblasts. ( E ) IL-4 concentration in the medium collected from ESCs, ESCs differentiating in EBs, and C2C12 myoblasts. Data are represented as means of three independent experiments with standard deviations.
Article Snippet: Additionally, ESCs and EBs were cultured in the presence of 5 ng/mL IL-4 and either 5 μg/mL or 10 μg/mL of anti-IL-4 antibody (I7034 Sigma Aldrich), or 5 ng/mL or 10 ng/mL of
Techniques: Expressing, Flow Cytometry, Negative Control, Staining, Derivative Assay, Concentration Assay